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KMID : 0350519950480020351
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Journal of Catholic Medical College
1995 Volume.48 No. 2 p.351 ~ p.362
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Changes in Antigenicity of Tissue-cyst Forming Strains of Toxoplasma gondii in Mouse
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Abstract
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Western blot and enzyme-linked immunosorbent assay (ELISA) were performed to clarify the differences and the changes of antigenicity according to the time course of infection with tissue-cyst forming strains of Toxoplasma gondii, Beverley,
Fukaya,
and
Me49 into mice. In both assays PH strain of T. gondii was used as antigen and the sera of 10 infected mice were reacted at 1, 2, 3, 4, 6, 8, 10, and 12 weeks post-infection.
@ES The results were as follows :
@EN 1. In the sera infected with Beverley strain, the major antigens were 23, 30, 90, 110, and 130 KDa bands until 12 weeks. The reactivity of antigen was more increased in 4 weeks after infection, and various bands were detected additionally.
2. In the sera infected with Fukaya strain, it was revealed the presence of major antigens at 23, 30, and 90 KDa. The 30 KDa band was detected more strongly than 23 KDa band band as early in 2 weeks. And in addition, 75 kDa band was especially
observed
from 3 weeks.
3. In the sera infected with Me49 strain, 23, 30 and 90 KDa bands were observed from 2 weeks, and 66 KDa band from 3 weeks especially.
4. The results of ELISA revealed that antigenicity of three strains increased in proportion to a linear equation such as y=0.084x-0.041 up to 4 weeks after infection. It was 0.036, 0.071 and 0.028 that the increment of absorbance in Beverley,
Fukaya,
and Me49 strain form 4 to 12 weeks after infection, respectively.
In this study, exclusive specific antigen in each strain was not found, but differences of antigenic reactivity were found in the course of time after infection. In spite of being the same tissue-cyst forming strain, there were differences of
antigenicity that induced immune response of host. And it was confirmed that western blot assay might be complementary to ELISA especially in the early phase of infection.
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KEYWORD
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